Plant secondary metabolites occupy a significant place in the pharmaceutical industry, constituting 25% of the medical prescriptions used in the central countries. The objective of this line of work is to develop phyto-enzymatic processes in bioreactors for the production of secondary metabolites in a continuous, reproducible and sustainable way with the environment through the combined application of metabolic engineering strategies, elicitation, in situ removal and hydrodynamic stress in crops. in vitro vegetables. In the same vein, the production of a) anthraquinones in cultures of Morinda citrifolia and Rubia tinctorum cellular suspensions and transformed roots and b) tropane alkaloids in Brugmansia candida transformed roots are approached.