A short peptide fragment of the vascular endothelial growth factor as a novel ligand for bevacizumab purification. Gabriela R Barredo et al., Protein Expr Purif .2019
Recientemente el grupo de Péptidos bioactivos y ligandos del Instituto de NANOBIOTEC y Colaboradores. han publicado el siguientes artículo:
A short peptide fragment of the vascular endothelial growth factor as a novel ligand for bevacizumab purification. Gabriela R.Barredo, Silvana L.Giudicessi, María C. Martínez Ceron, Soledad L. Saavedra, Santiago Rodriguez, Lucas Filgueira Risso, Rosa Erra-Balsells, Gustavo Mahler, Fernando Albericio Osvaldo Cascone y Silvia A. Camperi. Protein Expresion and Purification (2019). https://doi.org/10.1016/j.pep.2019.105500
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Highlights
- •A short peptide for bevacizumab purification was designed.
- •Bevacizumab was purified with an Ac-PHQGQHIGVSK-agarose chromatography column.
- •Mild conditions used during the chromatography preserves the full integrity of bevacizumab.
- •Maximum capacity of peptide-matrix was comparable to commercial protein A matrices.
- •The peptide ligand has a higher stability and a lower cost than protein A.
