Publicaciones recientes del grupo de diversidad microbiana del Instituto de Nanobiotec

Recientemente el grupo de diversidad microbiana del Instituto  de Nanobiotec ha publicado los siguientes trabajos:

Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate.  Kuc V ·  Vázquez SC, ·  Hernández E · Martinez‑Alvarez L, Villalba Primitz J, ·Mac Cormack WP, · Ruberto L (2019)  Polar Biology 42,  1157–1166. doi:10.1007/s00300-019-02508-1

Hydrocarbon contamination in soils from extremely cold areas, such as those from Antarctica, requires the development of specific remediation strategies for cleaning up anthropogenic pollution. Previous reports evidenced that after on-site biostimulation process of gasoil-contaminated Antarctic soils, 20% of the initial hydrocarbons remained undegraded (mainly C11–C14 n-alkanes). In the present work, these n-alkanes were added as sole carbon and energy source to enrichment cultures inoculated with the previously treated soil (biostimulation) as microorganism’s source to investigate changes occurring in the bacterial community structure. Three subcultures (8, 16, and 24 days) were performed from each enrichment culture. Changes in bacterial communities among different cultures and its subcultures were evidenced by Denaturing Gradient Gel Electrophoresis (DGGE). Results showed that even differences of one C in the alkane chain-length led to different community structures that evolved divergently from the original one. Clusters analysis showed that while samples grouped mainly by culture time, substrate-dependent differences were also evident. Isolation of biological tools for bioremediation from the cultures showed that Pseudomonadaceae members were omnipresent, whereas Rhodococcus spp. were obtained in cultures with the longest chain-length substrates. Results provided evidence about the presence of certain substrate preference of soil bacteria (even when substrates differed only in one C-atom of their chain-length), leading to different community structures. A collection of psychrotolerant hydrocarbon degrading/tolerant strains was obtained, representing a valuable tool for the design of a bioaugmentation strategy as a second, more specific stage, targeting the remnant hydrocarbons after a first bioremediation process involving biostimulation.

Antarctic Yeasts as a Source of Enzymes for Biotechnological Applications.  Martorell MM, Ruberto LAM, Castellanos de Figueroa LI,  Mac Cormack WP (2019) En:Fungi of Antarctica pp 285-304|Springer. Ed. LE Rosa. New York.  DOI: https://doi.org/10.1007/978-3-030-18367-7_13

Psychrophilic and psychrotrophic microorganisms present an enzymatic metabolism
of outstanding characteristics arising from the extraordinary environmental
challenges with which they must deal. This fact makes cold-adapted microorganisms
a relevant target for scientific research and its possible use in several industrial
processes (Cavicchioli et al. 2011). These enzymes present several advantages for
use in industrial applications. Among these advantages is their thermolability, which
makes them useful when a selective thermal inactivation step is necessary. In addition,
as they are synthesized at low temperatures, there is a potential reduction in
energy costs during their technological production and, additionally, their use in
processes occurring at low temperatures greatly reduces the chances of contamination
with mesophilic microorganisms (Javed and Qazi 2016)......Read more in:

DOI: https://doi.org/10.1007/978-3-030-18367-7_13