Seminario"Desarrollo y caracterización de una formulación mejorada de nanopartículas sólidas lípidas como sistema eficiente para el delivery de genes". Disertante: María José Limeres . Fecha: 25/9/2019 Horario: 12:15

Fecha: 25/9/2019

Horario: 12:15

Lugar: Cátedra de Biotecnología. FFyB. UBA. Junín 956 Piso 6. CABA
Aula: 602

Disertante: María José Limeres (IMPaM)

Tìtulo:"Desarrollo y caracterización de una formulación mejorada de nanopartículas sólidas lípidas como sistema eficiente para el delivery de genes".

Resumen:

The nanoparticle-mediated plasmid delivery is considered a useful tool to
introduce foreign DNA into the cells for the purpose of DNA vaccination
and/or gene therapy. Cationic solid lipid nanoparticles (cSLNs) are
considered one of the most promising non-viral vectors for nucleic acid
delivery. Based on the idea that the optimization of the components is
required to improve transfection efficiency, the present study aimed to
formulate and characterize cholesteryl oleate-containing solid-lipid
nanoparticles (CO-SLNs) incorporating protamine (P) to condense DNA so as
to produce P:DNA:CO-SLNs complexes as non-viral vectors for gene delivery
with reduced cytotoxicity and high cellular uptake efficiency.